Pad Fan

October 18th, 2011

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How do I incubate bacteria? What temp?

I am doing a science fair project on disinfecting water. I disinfect the water then take water samples and put in on a petri dish to see if it was disinfected properly and see if bacteria grows. It says to put it somewhere warm for it to grow but the house is at 78 degrees not warm enough. Outside is still to cold. Can i build a styrofoam incubator with a light bulb or heating pad, fan to move around heat, and a water heater thermostat for the temperature monitoring? I really dont want to spend $150 dollars for an incubator im going to use once. I have an infrared thermometer to measure the temp inside. What temperature to bacteria need to be at? And is any humidity required? How do i dispose of the bacteria after im done? Is extremely accurate temperature monitoring required or with 2-3 degrees off make a difference? Thanks

There are two things you should consider for culturing bacteria. The first one is the type of bacteria you are expecting. Water can be contaminated with coliforms ( for example E. coli). Once you disinfect the water, you should give a proper growing environment for the bacteria. You need sterile culture medium- petridish containing culture medium such as LB Agar- or a tube with sterile broth such as LB broth. The idea is to give nutrients and suitable pH for the bacteria. You can use heating bulbs as you are planning as long as you keep your plates protected from further bacterial contamination. The bacteria may grow in regular petridish without culture medium, but the growth may be very slow. Also, it would be difficult to appreciate the growth as compared to culture plates where you can count the number of colonies by naked eye. The ideal growth temperature is 37C

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